Method Scripts Queries without flanks Queries with flanks -------------------------------------------------------------------- Probalign do_probalignRNA4.pl do_probalignRNA.pl BLAST do_blast3_gengen.pl do_blast3.pl SSEARCH do_ssearch3_gengen.pl do_ssearch3.pl ClustalW do_clustalw_gengen.pl do_clustalw.pl HMMER NONE do_hmmer4.pl Each script takes in the directory name of the benchmark and an optional file suffix for multiple simultaneous runs. So if you want to run Probalign on the benchmark without query flanks type "perl do_probalignRNA.pl RNA-genome-only-divergent/" Make sure the executables are present in the current directory.